C.M.R. 06, 096, ch. 410, app 096-410-B - PREPARATION OF ODOR INTENSITY REFERENCING SCALE [1]

This document describes the preparation of a 5-point odor intensity referencing scale in general conformance with the static-scale method outlined in ASTM Standard E544- 10 "Standard Practices for Referencing Suprathreshold Odor Intensity," ASTM International, West Conshohocken, PA, 2010, DOI: 10.1520/E0544-10, www.astm.org. This document is not intended to address user health and safety practices associated with the preparation of the odor intensity referencing scale. The appropriate health and safety resources should be consulted, and recommended guidelines followed, during scale preparation.

The scale is prepared with the reference odorant n-butanol (n-BuOH) in accordance with the concentrations specified in Table 1. Supporting rationale for the selection of n-butanol as the reference odorant is summarized in Appendix X1 of ASTM E544.

Table 1:5-Point Odor Intensity Referencing Scale

Level	n-butanol Concentration in Water (ppm)
1 2 3 4 5	150 300 600 1200 2400

The scale ranges from 1 to 5.Level 1 consists of 150 parts permillion (ppm) of n-butanol in distilled water. Subsequent levels (Levels 2 through 5) increase by a factor of 2 based on a volume to volume basis.

Level 1 - Jar 1:150 ppm n-BuOH

Using a 1 milliliter (mL) pipet, measure 150 microliters (µL) of n-BuOH and add to 1,000 milliliter (mL) volumetric flask. Add distilled water to the 1,000 mL mark. Replace top and shake to mix. Use approximately 200 mL of the solution for Jar 1.

Level 2 - Jar 2:300 ppm n-BuOH

Using a 1 mL pipet, measure 300 µL of n-BuOH and add to 1,000 mL volumetric flask. Add distilled water to the 1,000 mL mark. Replace top and shake to mix. Use approximately 200 mL of the solution for Jar 2.

Level 3 - Jar 3:600 ppm n-BuOH

Using a 1 mL pipet, measure 600 µL of n-BuOH and add to 1,000 mL volumetric flask. Add distilled water to the 1,000 mL mark. Replace top and shake to mix. Use approximately 200 mL of the solution for Jar 3.

Level 4 - Jar 4:1200 ppm n-BuOH

Using a 1 mL pipet measure 1 mL of n-BuOH and add to 1,000 mL volumetric flask; next using a 1 mL pipet measure 200 µL of n-BuOH and add to the 1,000 mL volumetric flask for a total of 1200 µL of n-BuOH. Add distilled water to the 1,000 mL mark. Replace top and shake to mix. Use approximately 200 mL of the solution for Jar 4.

Level 5 - Jar 5:2400 ppm n-BuOH

Using a 2 mL pipet measure 2 mL of n-BuOH and add to 1,000 mL volumetric flask; next using a 1 mL pipet measure 400 µL of n-BuOH and add to the 1,000 mL volumetric flask for a total of 2400 µL of n-BuOH. Add distilled water to the 1,000 mL mark. Replace top and shake to mix. Use approximately 200 mL of the solution for Jar 5.

Notes

C.M.R. 06, 096, ch. 410, app 096-410-B